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Effect of NaHS on the hippocampal neuroinflammation of CUMS-exposed rats. Rats were exposed to CUMS for 4 weeks and treated with NaHS (30, 100 μmol/kg, i.p.) for 2 weeks. The levels of IL-4 (A) , IL-10 (B) , TNF-α (C) , IL-1β (D) , and IL-6 (E) in the hippocampus of rats were detected by <t>ELISA</t> kits. Values are expressed as mean ± SEM ( n = 3–5/group). *** P < 0.001, versus the control group; ### P < 0.001, versus the CUMS-exposed group.
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Effect of NaHS on the hippocampal neuroinflammation of CUMS-exposed rats. Rats were exposed to CUMS for 4 weeks and treated with NaHS (30, 100 μmol/kg, i.p.) for 2 weeks. The levels of IL-4 (A) , IL-10 (B) , TNF-α (C) , IL-1β (D) , and IL-6 (E) in the hippocampus of rats were detected by <t>ELISA</t> kits. Values are expressed as mean ± SEM ( n = 3–5/group). *** P < 0.001, versus the control group; ### P < 0.001, versus the CUMS-exposed group.
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Effect of NaHS on the hippocampal neuroinflammation of CUMS-exposed rats. Rats were exposed to CUMS for 4 weeks and treated with NaHS (30, 100 μmol/kg, i.p.) for 2 weeks. The levels of IL-4 (A) , IL-10 (B) , TNF-α (C) , IL-1β (D) , and IL-6 (E) in the hippocampus of rats were detected by <t>ELISA</t> kits. Values are expressed as mean ± SEM ( n = 3–5/group). *** P < 0.001, versus the control group; ### P < 0.001, versus the CUMS-exposed group.
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Effect of NaHS on the hippocampal neuroinflammation of CUMS-exposed rats. Rats were exposed to CUMS for 4 weeks and treated with NaHS (30, 100 μmol/kg, i.p.) for 2 weeks. The levels of IL-4 (A) , IL-10 (B) , TNF-α (C) , IL-1β (D) , and IL-6 (E) in the hippocampus of rats were detected by <t>ELISA</t> kits. Values are expressed as mean ± SEM ( n = 3–5/group). *** P < 0.001, versus the control group; ### P < 0.001, versus the CUMS-exposed group.
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Effect of NaHS on the hippocampal neuroinflammation of CUMS-exposed rats. Rats were exposed to CUMS for 4 weeks and treated with NaHS (30, 100 μmol/kg, i.p.) for 2 weeks. The levels of IL-4 (A) , IL-10 (B) , TNF-α (C) , IL-1β (D) , and IL-6 (E) in the hippocampus of rats were detected by ELISA kits. Values are expressed as mean ± SEM ( n = 3–5/group). *** P < 0.001, versus the control group; ### P < 0.001, versus the CUMS-exposed group.

Journal: Frontiers in Pharmacology

Article Title: Hydrogen sulphide alleviates depression-like behaviors by suppressing the hippocampal necroptosis-neuroinflammation-KP imbalance axis

doi: 10.3389/fphar.2025.1691204

Figure Lengend Snippet: Effect of NaHS on the hippocampal neuroinflammation of CUMS-exposed rats. Rats were exposed to CUMS for 4 weeks and treated with NaHS (30, 100 μmol/kg, i.p.) for 2 weeks. The levels of IL-4 (A) , IL-10 (B) , TNF-α (C) , IL-1β (D) , and IL-6 (E) in the hippocampus of rats were detected by ELISA kits. Values are expressed as mean ± SEM ( n = 3–5/group). *** P < 0.001, versus the control group; ### P < 0.001, versus the CUMS-exposed group.

Article Snippet: IL-4 ELISA kit (CSB-E04635r) and IL-10 ELISA kit (CSB-04595r) were purchased from CUSABIO.

Techniques: Enzyme-linked Immunosorbent Assay, Control

Effect of AAV-RIPK3 on NaHS-inhibited neuroinflammation in the hippocampus of CUMS-exposed rats. The levels of IL-4 (A) , IL-10 (B) , TNF-α (C) , IL-1β (D) , and IL-6 (E) in the hippocampus of rats were detected by ELISA kits. ** P < 0.01, *** P < 0.001 versus the vector group; # P < 0.05, ### P < 0.001, versus co-treated with CUMS and vector group; & P < 0.05, && P < 0.01, &&& P < 0.001, versus co-treated with CUMS, NaHS, and vector group.

Journal: Frontiers in Pharmacology

Article Title: Hydrogen sulphide alleviates depression-like behaviors by suppressing the hippocampal necroptosis-neuroinflammation-KP imbalance axis

doi: 10.3389/fphar.2025.1691204

Figure Lengend Snippet: Effect of AAV-RIPK3 on NaHS-inhibited neuroinflammation in the hippocampus of CUMS-exposed rats. The levels of IL-4 (A) , IL-10 (B) , TNF-α (C) , IL-1β (D) , and IL-6 (E) in the hippocampus of rats were detected by ELISA kits. ** P < 0.01, *** P < 0.001 versus the vector group; # P < 0.05, ### P < 0.001, versus co-treated with CUMS and vector group; & P < 0.05, && P < 0.01, &&& P < 0.001, versus co-treated with CUMS, NaHS, and vector group.

Article Snippet: IL-4 ELISA kit (CSB-E04635r) and IL-10 ELISA kit (CSB-04595r) were purchased from CUSABIO.

Techniques: Enzyme-linked Immunosorbent Assay, Plasmid Preparation